DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes
Identifieur interne : 000F31 ( Main/Exploration ); précédent : 000F30; suivant : 000F32DNA hydrolysis and voltammetric determination of guanine and adenine using different electrodes
Auteurs : N. Zari [Maroc] ; H. Mohammedi [Maroc] ; A. Amine [Maroc] ; M. M. Ennaji [Maroc]Source :
- Analytical letters [ 0003-2719 ] ; 2007.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Or.
English descriptors
- KwdEn :
Abstract
This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.
Affiliations:
Links toward previous steps (curation, corpus...)
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- to stream PascalFrancis, to step Curation: 000025
- to stream PascalFrancis, to step Checkpoint: 000036
- to stream Main, to step Merge: 000F37
- to stream Main, to step Curation: 000F31
Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acid hydrolysis</term>
<term>Adenine</term>
<term>Biosensor</term>
<term>Carbon electrode</term>
<term>Chemical sensor</term>
<term>DNA</term>
<term>Electrochemical method</term>
<term>Gold</term>
<term>Guanine</term>
<term>Oxidation</term>
<term>Paste electrode</term>
<term>Room temperature</term>
<term>Sensitivity</term>
<term>Square wave</term>
<term>Trace analysis</term>
<term>Voltammetry</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Hydrolyse acide</term>
<term>Voltammétrie</term>
<term>Biodétecteur</term>
<term>Onde carrée</term>
<term>Méthode électrochimique</term>
<term>Oxydation</term>
<term>Electrode carbone</term>
<term>Electrode pâte</term>
<term>Sensibilité</term>
<term>Analyse trace</term>
<term>Température ambiante</term>
<term>Capteur chimique</term>
<term>DNA</term>
<term>Guanine</term>
<term>Adénine</term>
<term>Or</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Or</term>
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<front><div type="abstract" xml:lang="en">This work reports the development of a biosensor method for the label-free detection of specific DNA sequences. In the initial phase, square wave voltammetry (SWV) was used in a comparative investigation into the electrochemical oxidation of purines (guanine and adenine) and DNA fragments at various electrode surfaces: carbon paste (CPE), glassy carbon electrode (GCE), and gold (AuE). Relative to the carbon electrodes, an approximate 4.0-fold, 6.0-fold, and 3.25-fold increase in the anodic response was observed when guanine, adenine, and hydrolyzed DNA, respectively, were measured on the AuE. It was shown that the guanine and adenine bases could be successfully determined by use of SWV for a deoxyribonucleic acid sample following acid hydrolysis. This label-free detection of hydrolyzed DNA on gold electrodes has significant advantages over methods using existing carbon electrode materials because of its higher sensitivity and the potential applicability of microfabrication techniques for the production of the requisite gold electrodes. In another phase of development, the times and conditions for DNA hydrolysis and purine release were investigated. It was shown that under optimal conditions, trace levels of the purine bases could be readily detected following 20 min of hydrolysis at room temperature. The proposed method can be used to estimate the guanine and Finally, when appropriate probe sequences were first adsorbed on the surface of the screen-printed gold electrode (SPGE), this electrochemical biosensor could be used to specifically detect sequences from ss corona virus aviair following hybridization and hydrolysis reactions on the sensor surface. No enhancement of the voltammetric response was observed when the sensor was challenged with a non-complementary DNA sequence.</div>
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